img

QQ群聊

img

官方微信

高级检索

黄金科学技术 ›› 2018, Vol. 26 ›› Issue (1): 115-123.doi: 10.11872/j.issn.1005-2518.2018.01.115

• 冶炼技术与装备研发 • 上一篇    下一篇

初始pH值对ASH-07细菌生长和黄铜矿浸出过程的影响

潘颢丹1,2,杨洪英2,佟琳琳2,李承卓1,杜世麟1,韩馨怡1,韩爽1   

  1. 1.辽宁石油化工大学,辽宁 抚顺 113001;
    2.东北大学,辽宁 沈阳 110819
  • 收稿日期:2017-07-11 修回日期:2017-11-21 出版日期:2018-02-28 发布日期:2018-05-19
  • 作者简介:潘颢丹(1983-),女,辽宁沈阳人,讲师,从事微生物稠油降黏研究工作。panhaodan@126.com
  • 基金资助:

    国家自然科学基金项目“含砷复杂金矿细菌氧化矿物界面能激起作用机制的基础研究”(编号:U1608254)、“难浸含砷金矿细菌氧化过程[AsS]2-→As(Ⅲ)→As(Ⅴ)转化机理研究”(编号:51374066)、辽宁省教育厅科学研究一般项目“生物表面活性剂在稠油讲解中的应用研究”(编号:L2016016),2016年大学生创新创业训练计划项目“数目类生物质的干燥实验研究”(编号:2016071)、“秸秆生物质干燥实验研究”(编号:2016079)和2017年大学生创新创业训练计划项目“生物质物料干燥和模型的建立”(编号:2017068)、“鸡类固废生物质的干燥箱实验研究”(编号:2017075)联合资助
     

Effects of Initial pH on ASH-07 Bacterial Growth and Chalcopyrite Bio-leaching Process

PAN Haodan 1,2,YANG Hongying 2,TONG Linlin 2,LI Chengzhuo 1,DU Shilin 1,HAN Xinyi 1,HAN Shuang 1   

  1. 1.Liaoning Shihua University,Fushun    113001,Liaoning,China;2.Northeastern University,Shenyang    110819,Liaoning,China
  • Received:2017-07-11 Revised:2017-11-21 Online:2018-02-28 Published:2018-05-19

摘要:

影响黄铜矿细菌浸出过程的因素很多,重点研究了初始pH值对浸矿细菌ASH-07生长活性、黄铜矿浸出效果和黄铜矿表面氧化膜成分的影响。不同初始pH值条件下细菌培养和黄铜矿浸出实验结果表明,在初始pH=1.2~1.8的条件下,细菌培养24 h后,即开始进入生长平衡期,浓度迅速升高至1.4×108 cell/mL,黄铜矿在浸出6 d后浸出率即达到45.0%左右;氧化膜成分XPS检测实验结果表明,在初始pH值为1.2的条件下,黄铜矿浸出168 h后,矿物表面氧化膜成分中未检测到钝化膜黄钾铁矾。因此,在采用浸矿菌种ASH-07浸出黄铜矿的实验中,最佳的初始pH值约为1.2,pH值低于1.2或高于1.8都不利于黄铜矿的生物浸出。

关键词: 初始pH值, 细菌生长, 黄铜矿, 生物浸出, 氧化膜, X射线光电子能谱(XPS)

Abstract:

Many factors affect the leaching processes of chalcopyrite.The effect of initial pH value on the activity of leaching bacteria ASH-07,the efficiency of the leaching result and compositions of the oxide film on the chalcopyrite surface were investigated.The results showed that the bacteria reached equilibrium phase and boomed to 1.4×108 cell/mL after cultured for 24 h on the condition of pH=1.2~1.8 after leaching for 6 d.The leaching efficiency rapidly reached approximately 45.0%.XPS analysis of the oxide film presented that no jarosite passive film was observed on the mineral surface after leaching for 168 h on the condition of initial pH=1.2. Therefore,the best initial pH value of approximately 1.2 was obtained for the chalcopyrite leaching with the leaching bacteria of ASH-07.It was not benefit for the bioleaching of the chalcopyrite when the pH value was lower than 1.2 or higher than 1.8.

Key words: initial pH value, bacteria growth, chalcopyrite, bioleaching, oxide film, X-ray Photoelectron Spectroscopy(XPS)

中图分类号: 

  • TF811
[ 1 ] Smith R W,Misra M,Dubel J. Mineral bioprocessing and the future[J].Minerals Engineering,1991,4(7-11):1127-1141.
[ 2 ] Ehrlich H L. Past,present and future of biohydrometallurgy[J].Hydrometallurgy,2001,59(2-3):127-134.
[ 3 ] Acevedo F. Present and future of bioleaching in developing countries[J].Electronic Journal of Biotechnology,2002,5(2):196-199.
[ 4 ] Brierley J A.A perspective on developments in biohydrometallurgy[J].Hydrometallurgy,2008,94(1/2/3/4):2-7.
[ 5 ] Marhual N P,Pradhan N,Kar R N,et al. Differential bioleaching of copper by mesophilic and moderately thermophilic acidophilic consortium enriched from same copper mine water sample[J].Bioresource Technology,2008,99(17):8331-8336.
[ 6 ] Watling H R. The bioleaching of sulphide minerals with emphasis on copper sulphides- A review[J].Hydrom etal lurgy,2006,84(1/2):81-108.
[ 7 ] Lakshmi R S,Fayazuddin Md,Ray L F,et al. Electron paramagnetic resonance and optical absorption spectral studies on chalcocite[J].Molecular and Biomolecular Spectroscopy,2007,68(3):420-423.
[ 8 ] Sowjanya G,Reddy N C G,Lakshmi R S,et al. Electron paramagnetic resonance and optical absorption spectral studies on covellite mineral[J].Molecular and Biomolecular Spectroscopy,2008,71(3):751-754.
[ 9 ] Fuentes G,Viñals J,Herreros O. Hydrothermal purification and enrichment of Chilean copper concentrate Part 1:The behavior of bornite,covellite and pyrite[J].Hydrom etal lurgy,2009,95(1/2):113-120.
[10 ]  Rohwerder T,Gehrke T,Kinzler K,et al. Bioleaching review part A:Progress in bioleaching:fundamentals and mechanisms of bacterial metal sulfide oxidation[J].Applied Microbiology and Biotechnology,2003,63(3):239-248.
[ 11 ] Vilcáez J,Yamada R,Inoue C. Effiect of pH reduction and ferric ion addition on the leaching of chalcopyrite at thermophilic temperatures[J].Hydrom etal lurgy,2009,96(1/2):62-71.
[ 12 ] C?rdoba E M,Muñoz J A,Blázquez M L,et al. Leaching of chalcopyrite with ferric ion.Part I:General aspects[J].Hydrometallurgy,2008,93(3/4):81-87.
[ 13] ] Antonijevi? M M,Bogdanovi? G D. Investigation of the leaching of chalcopyrite ore in acidic solutions[J].Hydrom etal lurgy,2004,73(3/4):245-256.
[ 14] ] 温建康,姚国成,陈勃伟,等. 溶液pH值对浸矿微生物活性及浸出速率的影响研究[J].稀有金属,2009,33(1):80-83.Wen Jiankang,Yao Guocheng,Chen Bowei,et al. Effect of pH on activities of mineral-bioleaching micro-organisms and bioleaching rate[J].Chinese Journal of Rare Metals,2009,33(1):80-83.
[ 15] ] Okibe N,Johnson D B. Biooxidation of pyrite by defined mixed cultures of moderately thermophilic acidophiles in pH-controlled bioreactors:Significance of microbial interactions[J].Biotechnology and Bioengineering,2004,87(5):574-583.
[ 16] ] Kumar R N,Nagendran R. Influence of initial pH on bioleaching of heavy m etal s from contaminated soil employing indigenous Acidithiobacillus thiooxidans[J].Chemosphere,2007,66(9):1775-1781.
[ 17 ] 潘颢丹. 黄铜矿细菌浸出机理研究及其过程控制[D].沈阳:东北大学,2012.Pan Haodan. Study on the Mechanism of Bacterial Leaching of Chalcopyrite and Its Process Control[D].Shenyang:Northwestern Universtiy,2012.
[ 18 ] 潘颢丹,杨洪英,陈世栋,等. 不同条件下黄铜矿表面钝化现象的研究[C]//中国有色金属学会第八届学术年会论文集.北京:中国有色金属学会,2010:146-151.Pan Haodan,Yang Hongying,Chen Shidong,et al. Study on the surface passivation phenomenon of chalcopyrite under different conditions[C]//Symposium of Eighth Annual Conference of the Nonferrous Metals Society of China.Beijing:The Nonferrous Metals Society of China,2010:146-151.
[ 19] ] Silverman M,Lundgren D. Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans:I.An improved medium and a harvesting procedure for securing high cell yields[J].Journal of Bacteriology,1959,77(5):642-647.
[ 20 ] 沈萍,范秀容,李广斌. 微生物学实验[M].北京:高等教育出版社,2001:90-92.Shen Ping,Fan Xiurong,Li Guangbin. Microbiology Experiment[M].Beijing:Higher Education Press,2001:90-92.
[ 21 ] 符斌,李华昌. 有色冶金分析手册[M].北京:冶金工业出版社,2004.Fu Bin,Li Huachang. Analysis Manual of Nonferrous M etal lurgy[M].Beijing:Metallurgical Industry Press,2004.
[ 22] ] 杨洪英,潘颢丹,佟琳琳,等. 黄铜矿晶体表面生物氧化膜的形成过程[J].金属学报,2012,48(9):1145-1152.Yang Hongying,Pan Haodan,Tong Linlin,et al. Formation process of biological oxide film on chalcopyrite crystal surface[J].Acta Metallurgica Sinica,2012,48(9):1145-1152.
[ 23] ] Ahonen L,Tuovinen O H. Bacterial leaching of complex sulfide ore samples in bench-scale column reactors[J].Hydrom etal lurgy,1995,37(1):1-21.
[ 24 ] Liu J S,Li B M,Zhong D Y,et al. Preparation of jarosite by Acidithiobacillus ferrooxidans oxidation[J].Journal of Central South University of Technology,2007,14(5):623-628.
 
No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!